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D1315
Sigma
DT Diaphorase human
lyophilized powder, recombinant, expressed in Escherichia coli
| Synonym: | DTD, NQO1, Quinone reductase |
| Enzyme Commission (EC) Number: | 1.6.99.2 ( BRENDA | IUBMB ) |
| MDL number: | MFCD00130947 |
Description
| Biochem/physiol Actions | Shown to activate quinone based anti-tumor agents in vivo. Suitable for conjugation to carrier molecules. |
| Unit Definition | One unit will reduce 1.0 μmole cytochrome C per min/mg in the presence of menadione substrate at 37 °C. |
Properties
| recombinant | expressed in Escherichia coli |
| form | lyophilized powder |
| activity | ≥100 units/mg protein |
| mol wt | monomer mol wt 31000 |
| matrix | 2% Lactose and 8% PBS buffer salts. Does not contain BSA as excipient. |
| Gene Information | human ... NQO1(1728) |
| storage temp. | 2-8°C |
Safety
| Personal Protective Equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
| WGK Germany | 3 |
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References
| reference | Doughtyl, S.W. and Phillips, R.M., Molecular of Modelling of Human DT-Diaphorase for Enzyme-Directed Bioreductive Drug Design. Mol. Simul. 24, 209, (2000) |
| Phillips R.M., et al., Bioreductive activation of a series of indoloquinones by human DT-diaphorase: Structure-activity relationships. J. Med. Chem. 42, 4071, (1999) |
